Background: Amblyopia is defined as uniocular or binocular decreased best corrected visual acuity without any identifiable pathology in the eye or visual pathway. Occlusion therapy is the main stay of treatment in these cases. Visual acuity is often improved if the disease is detected at earlier age and if the patient is well compliant to therapy. It is observed that there is some compromised contrast sensitivity in some patients even after improvement of visual acuity which renders this patients unsatisfactory. Objective: To assess the status of contrast sensitivity after occlusion therapy in patients of anisometropic amblyopia. Methodology: This prospective observational study was conducted over 50 diagnosed patients of anisometropic amblyopia in the department of Paediatric Ophthalmology in National Institute of Ophthalmology & Hospital from January, 2021 to December, 2022. Patients fulfilling the selection criteria were enrolled in the study. Selected patients underwent detail ophthalmic and systemic evaluation as well as relevant investigation. Visual acuity was assessed with special emphasis which includes unaided, with pinhole and after refraction. Visual acuity was recorded in Snellel’s chart and converted into Log MAR unit. Contrast sensitivity was tested monocularly on both eyes in all the study subjects by Pelli–Robson chart. Occlusion of better eye was done in all patients. Duration of occlusion depends on presenting best corrected visual acuity (Worse than 6/60:6 waking hours,6/60-6/24:4 waking hours and 6/18-6/12:2 waking hours). They were followed up 1 months after starting occlusion therapy. Occlusion therapy was continued when there is stable vision for 6 months or vision improves better than 6/12. Data were collected 1 month, 3 months and 6 months after starting occlusion therapy. Mean score of visual acuity (Log MAR unit) was taken from available data at that time and was compared with the baseline data. Contrast acuity was assessed in all patients by Pelli–Robson chart during final follow-up. Mean value of the contrast acuity was compared between before and after occlusion therapy. All the relevant data were recorded in pre-designed data collection sheet and analyzed by window software SPSS ver.21. Results: In this study the mean age of the study subjects were 8±1.72 years, out of them, 23 were male and 27 were female. Baseline mean best- corrected visual acuity in Log MAR unit was 0.70±0.15 (SD). It was 0.55±0.13 (SD),0.49±0.09 (SD) and 0.37±0.11(SD) in 1st month, 3rd month and 6th month respectively. Contrast sensitivity by Pelli-Robson chart of the study subjects. The base line contrast sensitivity was 1.19±0.20 (SD) and it was 1.18±0.20 (SD) during final follow-up. Conclusions: Quantitative assessment and comparison of mean contrast acuity after occlusion therapy with presenting acuity showed that there was no significant changes in contrast acuity after occlusion therapy though the improvement of mean visual acuity.